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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been observed in many tumors, which results in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was discovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been described but in depth nucleotide sequence information is just not however out there.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly includes the epitope regarded by the antibody.
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This tutorial outlines additional possible optimization of mobile and immunoassay parameters to be certain the best possible success are...
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This tutorial outlines additional attainable optimization of mobile and immunoassay parameters to ensure the best possible benefits are...
Antibodies sure to the blocking peptide now not bind into the epitope around the target protein. By comparing the staining in the blocked antibody versus the click here antibody alone, you can see which staining is unique.
Target Information Cyclin E belongs to the extremely conserved cyclin relatives, whose customers are characterised by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs which contribute to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and features as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary which is degraded as cells progress by S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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